Akt Vipolnennih Rabot Ks2 Blank Kazahstan
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• Western blot analysis of recombinant Akt1, Akt2 and Akt3 proteins, and extracts from HeLa, C2C12, C6 and COS cells, using Akt (pan) (11E7) Rabbit mAb. • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Akt (pan) (11E7) Rabbit mAb in the presence of control peptide (left) or Akt (pan) (11E7) Blocking Peptide #1085 (right). Halid ziya ushakligilj zapretnaya lyubovj knigu md. • Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Akt (pan) (11E7) Rabbit mAb. • Immunohistochemical analysis of paraffin-embedded human melanoma, using Akt (11E7) Rabbit mAb. • Immunohistochemical analysis using Akt (pan) (11E7) Rabbit mAb on SignalSlide(TM) Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right)). Note the lack of phosphorylated Akt-associated stain at the membrane of the LY294002 treated cells.
• Immunohistochemical analysis of paraffin-embedded HeLa cells untreated (left) or transfected with Akt 1/2/3 Kinases ShortCut® siRNA Mix (New England BioLabs #N2005) (right), using Akt (pan) (11E7) Rabbit mAb (top) or Cleaved Caspase-3 (Asp175) #9661 (bottom). Note the induction of cleaved caspase-3 in Akt deficient cells.
• Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or insulin-treated (right), using Akt (pan) (11E7) Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
• Flow cytometric analysis of untreated Jurkat cells, using Akt (pan) (11E7) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). Western Blotting Protocol For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween ® 20 at 4°C with gentle shaking, overnight. NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution. Grinev s v vvedenie v terminovedenie 1993. Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Western Blotting Application Solutions Kit NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water. • 20X Phosphate Buffered Saline (PBS): () To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH 2O, mix. • 10X Tris Buffered Saline (TBS): () To prepare 1 L 1X TBS: add 100 ml 10X to 900 ml dH 2O, mix. • 1X SDS Sample Buffer: Blue Loading Pack () or Red Loading Pack () Prepare fresh 3X reducing loading buffer by adding 1/10 volume 30X DTT to 1 volume of 3X SDS loading buffer.